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KMID : 0362220130400020143
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Journal of The Korean Research Society for Dental Materials
2013 Volume.40 No. 2 p.143 ~ p.151
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Effects of Bis-GMA and HEMA on the cytotoxicity of dentin bonding agents
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Park Hyun-Jae
Son Kyung-Mi
Kwon Ji-Hyung
Park Hee-Chul
Zhu Ting Ting
Yang Hyeong-Cheol
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Abstract
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It has been reported that dentin bonding agents (DBA) used in composite resin adhesion have a strong cytotoxicity. Bisphenol A glycidyl methacylate (Bis-GMA) and 2-hydroxyethyl methacrylate (HEMA) are known as the main ingredients in a large number of resin-based DBA. In this study, we quantified Bis-GMA and HEMA in dentin bonding agents by using high performance liquid chromatography (HPLC), and investigated whether these resin monomers were responsible for the cytotoxicity of DBA. In 1 ㎎ of Adper Single Bond 2, Clearfil-SE Bond Adhesive and Adper Scotchbond Multi-Purpose Adhesive, 0.3475, 0.4899 and 0.2717 ㎎ of Bis-GMA were detected, respectively, and 0.1475, 0.3092 and 0.4372 ㎎ of HEMA were detected, respectively. Based on the results of the cytotoxicity, Bis-GMA is likely a major cause of cytotoxicity. In 1㎎ of Clearfil-SE primer, Adper Scotchbond Multi-Purpose Primer, One-UP Bond F plus A and One-UP Bond F plus B, 0.1377, 0.6099, 0.1024 and 0.8595 ㎎ of HEMA were detected respectively. Bis-GMA was not detected in those DBA (detection limit: 5 ㎛). Based on the results of the cytotoxicity, the cytotoxicity of Clearfil-SE Bond Primer is expected due to HEMA. Adper Scotchbond Multi-Purpose Primer showed low cytotoxicity despite the high amount of HEMA. Therefore, it is assumed that the components that exhibit the antagonic effect on the cytotoxicity of HEMA are included in Adper Scotchbond Multi-Purpose Primer. The result of One-UP Bond F plus B showed that HEMA is a major cause of cytotoxicity. But, in case of One-UP Bond F plus A, other cytotoxic ingredients rather than HEMA likely exist. Bis-GMA and HEMA was not detected in Prime & Bond NT, I Bond and G-Bond, in which the other cytotoxic ingredient was assumed included.
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KEYWORD
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Dentin bonding agent, Bis-GMA, HEMA, cytotoxicity, HPLC
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